Abstract

ABSTRACT: Roses have been one of the world’s most popular ornamental plants for a long time. Recently, in vitro flower induction in roses was demonstrated. To reduce the intensive labour requirement along with the production cost during plant propagation by tissue culture technique, there is an immense need of developing scale-up system and automation. Progress in tissue culture automation will depend upon the use of liquid culture in bioreactors. This system usually involves a wetting and drying cycle which occur periodically in a given period of time. Present investigation was carried out to determine the efficiency of in vitro propagation of Rosa hybrida cv. Black baccara with tow type periodical bioreactor. For mass propagation in bioreactors, 7-10 nodal segments were transferred into bioreactors containing VS liquid medium supplemented with 3% sucrose and 2 μM 6-Benzylaminopurine (BAP). The nutrition cycle was set at 10 min every 24h. The results showed that the semi- continuous system is more benefit than routine system (250ml jars) for shoot number, shoot length and fresh/dry weight. Comparative studies between two types periodical bioreactor indicated that there was not significant difference between the performances of two types of bioreactors. Plants produced in Bioreactor had better grown (3.39 and 0.57 g for fresh and dry weight, respectively) than plants which grown in 250 ml jars (0.56 and 0.02 g for fresh and dry weight, respectively). Also these kinds of bioreactor could be used for mass propagation with reduced cost, energy and handle work. Key words: In vitro, Liquid media, Periodical Bioreactor, Rose, Shoot multiplication.