view:21356 Last Update: 2016-9-26
Mohammad E. Amiri* and Someih Gharati
Influence of medium composition on multiplication of walnut (Juglans regia L.) growth
In vitro proliferation of walnut (Juglans regia var. zeiabadi) was studied in response to the four levels
(1/3X, 1/2X, 1.0X and 1.5X) of macrominerals of DKW (Driver and kunjyukj, 1984) medium.
Macrominerals (N, P, K, Mg) can be a limiting factor for growth quantity (fresh weight and dry weight)
and growth quality (explants appearance) of walnut explants. Addition of 0.5X macrominerals to the
basal DKW medium in which containing 4.0 µM 6-benzylaminopurine (BA) and 12 µM indole-3-butyric
acid (IBA), significantly (P=0.05) improved the proliferation. Better response of was found with 1.5X
macrominerals concentration in respect of growth and proliferation. In contrary, the decrease in
mineral availability observed in the depressed growth in the low macrominerals supply treatments (1/3X
and 1/2X), (the restricted mineral availability was the main cause of the inhibition of the growth).
However, root formation was worsted by supplemented additional minerals. For example, low mineral
concentration treatment exhibit high rooting ability (75%) and high mineral concentration showed low