Abstract

This research was carried out to evaluate the response of seven of selected Persian leek accessions to in vitro gynogenesis. The experimental design was factorial based on completely randomized design with three culture medium B2D0 (2 mgl-1 BA), B2D2 (2 mgl-1 BA and 2 mgl-1 2, 4-D) and B2D4 (2 mgl-1 BA and 4 mgl-1 2, 4-D) and seven Persian leek accessions (Gorgan, Neishabur, Hamedan, Mianeh, Kangavar, Varamin and Kerman) with three replications. Traits of embryo rate, regeneration rate, survival rate, callus formation and vitrified flowers have evaluated. In this experimental from a total of 18,900 flower buds cultured, 459 embryos (2.43%), of which all successfully regenerated to complete plants. Gorgan and Mianeh accessions showed the highest embryo and regeneration rate (25%) in culture medium B2D2 (2 mgl-1 BA and 2 mgl-1 2, 4-D). Results showed that unpollinated flower culture is an effective method to in vitro gynogenesis of Persian leek. Keywords: Persian leek, Gynogenesis, In vitro culture and Unpollinated flower culture.