view:14905 Last Update: 2023-1-8
Pooria Khademi, Javad Moghaddam, Rozbeh Khademi, Saeid Veradi Esfahani, Esmaiel Shahabi
Creating Anti-HIV-Infection effect by Synthesis of AuNPs from Rosa canina L. Fruit Extract
ایجاد اثر ضد بیماری ایدز توسط سنتز AuNPs نانوذرات طلا از میوه گیاه Rosa canina L.
Background and objective: Rosa canina fruit extract has evident immunomodulatory properties. The objective of this study was to produce AuNPs using this extract and to compare their anti-HIV effects with AuNPs produced through the conventional Turkevich method. Method: In this study, AuNPs were produced through Turkevich method and through reduction using Rosa canina fruit extract. They were characterized through TEM, DLS, UV-visible and FT-IR analyses and their cytotoxicity was evaluated through MTT assay. After HIVs were separated from PBMCs of the HIV-infected human body, they were exposed to interaction with both types of nanoparticles. p24 antigen test was utilized to obtain and compare the percent inhibition of p24 antigen, as a symbol of anti-HIV activity of each type. Results: In contrast with AuNPs produced through the conventional Turkevich method, nanoparticles produced with the extract had better size stability and preserved their average size of 27.7 nm in physiological condition. For nanoparticles produced with the extract, when the viruses interacted with the nanoparticles and then infected the healthy PBMCs, the percent inhibition of p24 antigen was 61%; but when the PBMCs were infected by the viruses and then exposed to these nanoparticles, the percent inhibition was 53%. Conclusion: Rosa canina fruit extract can act as a novel and potent reducing and stabilizing agent for the synthesis of AuNPs. The new nanoparticles exhibit better anti-HIV effects compared to nanoparticles produced through the conventional Turkevich method. The novel nanoparticles produce virucidal and inhibitory effects by occupying attachment sites and preventing the virus from binding with T-lymphocyte, or modifying proteins involved in HIV replication.