Abstract

Graphene is a two-dimensional Carbon allotrope which is made out of carbon atoms arranged on a hexagonal lattice. Graphene oxide is an applicable precursor to synthesis graphene-based new nanomaterials. Many parameters may affect their biological interactions with cells, and the concentration of GO nanosheets is one of the important factors. In this work , graphite was oxidized by the modified Hummers method, then dispersed by ultrasonic waves (supersonic wave shock). The prepared samples were characterized by ultraviolet-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. Antibacterial tests have been performed for various concentration of graphene oxide for both Gram-positive and Gram-negative bacteria. Results show that the antibacterial activity of this paper is dependent on graphene oxide concentration. By increasing the graphene oxide concentration (upto 800 μg/mL) the cell inactivation rate increases. In addition, results show that graphene oxide nanosheets are more toxic to Gram-negative bacteria than Gram-positive bacteria.