Abstract

Dog rose ( as rootstock for ornamental roses as concentrations of NH 10:35, 15:30, 15:35, 15:40, 20:35 and 20:45) was investigated on proliferation stage of dog rose cultivated on Ms media with total nitrogen levels (35; 40, 45, 50, 55, 60 and 65 mM). The relative amounts of NH + - numbers were obtained with 20:45 NH + - 20:45 NH + - - + produced more and chlorosis leaf number was decreased. This study also demonstrates that with increasing nitrogen levels, the growth of shoots and node numbers were more but leaf chlorosis was decreased, therefore proliferation was better. The percentage of axillary shoot was affected by NH Rosa canina) is one of the most important ornamental and medicinal plants which are usedRosa hybrida and Rosa floribunda. The effects of different4NO3 (10-15 and 20 mM), KNO3 (15-20 and 25 mM) and NH4:NO3 ratios (10:25, 10:30,4and NO3ions influenced the tested parameters. The highest shoot length and most node4:NO3ratios. Chlorosis leaf number was less with 15:35 and4:NO3ratios. When NO3was higher than NH4, shoot length and node numbers were4NO3 concentrations and it was highest in 20 mM concentration, but with increasing KNO and feeble callusing percentage at the cut ends of explants were more and lower in row. Key words:   3, the leaf necrotic Dog rose, in vitro culture, MS nitrogen source, NH4 +: NO3 - ratio, proliferation.