Abstract

In this study, biodegradable nanocomposite film composed of pullulan – whey protein isolate (WPI) – montmorillonite (MMT) were developed and characterized as a function of incorporating various amounts of MMT nanoparticles (0, 1, 3 and 5% wt). Results showed that the water-vapor permeability, moisture content, moisture absorption and water solubility decreased when the nano-MMT content was increased. Tensile strength improved and elongation at break simultaneously decreased with increasing MMT content. The glass transition temperature (Tg( and melting-point temperature (Tm)  increased with increasing nano-MMT content. Scanning electron microscope (SEM) and X-ray diffraction (XRD) analysis revealed uniform distribution of MMT into the polymer matrix. Atomic force microscopy (AFM) showed enhancement of films' roughness with increasing MMT content.